Molecular Mechanisms of Protective Effect of Resveratrol Against Cisplatinium Induced Ototoxicity

neurotoxicity limit to reach high doses. It mainly induces apoptosis in auditory sensory cells. Resveratrol is a polyphenol mostly found in grape skin and seed. It has anti-oxidant, neuroprotective and dose dependent antiapoptotic properties. It was claimed to protect side effects of some chemotherapeutic agents. In this study possible otoprotective effects of resveratrol were studied in House Ear Institute-Organ of Corti 1 cell line. Materials and Methods: Cells were exposed to cisplatinium, resveratrol and their combination for 24 hours at different doses. Cell viability analysis, apoptosis and expression of 84 apoptosis related genes were determined in pre-optimized doses. Results: Resveratrol pretreatment of the cells before cisplatinium led to 33% decrease in apoptotic cell count when they were compared with controls. Expression of apototic genes was decreased with cisplatinium and resveratrol combination. Conclusion: This study revealed that resveratrol in low doses had an in vitro protective effect on cisplatinium ototoxicity mainly working on apoptotic gene expressions, but it has cytotoxic effect in high doses.